A GUID (Globally Unique Identifier) is a 128-bit unique identifier used in software applications to ensure uniqueness across systems and databases. GUIDs are commonly used in distributed computing, database records, API keys, and cryptographic applications where unique identification is required. GUIDs are typically represented as a 32-character hexadecimal string, often formatted with hyphens for readability. They can be generated using various algorithms, including UUID versions 1, 3, 4, and 5, with version 4 being the most commonly used, as it relies on randomness. GUIDs help prevent data collisions, maintain integrity, and are widely used in operating systems, cloud computing, and web services.